Expression of serum and glucocorticoid-regulated protein kinase 3 in breast cancer stem cells

doi:10.3969/j.issn.2095-4344.2013.40.007

Abstract

Abstract:

BACKGROUND: Serum and glucocorticoid-regulated protein kinase 3 may be related to tumor progression, and can prevent the apoptosis of breast cancer cells induced by interleukin-3 withdrawal. Serum and glucocorticoid regulated protein kinase 3 possibly participate in the regulation of intracellular Wnt signal pathway.
OBJECTIVE: To investigate the expression of serum and glucocorticoid-regulated protein kinase 3 and β-catenin protein expression in breast cancer cells.
METHODS: Breast cancer stem cells were isolated, identified and subcultured from 71 breast cancer cases by dual-wave flow cytometry. The 30 normal breast tissues were used as the controls. SP immunohistochemistry analysis was used to detect the level of serum and glucocorticoid-regulated protein kinase 3 and β-catenin protein expression in breast cancer stem cells and normal breast tissues.
RESULTS AND CONCLUSION: The expression of serum and glucocorticoid-regulated protein kinase 3 and β-catenin in breast cancer stem cells were both higher than that of normal breast tissues (P < 0.01). There had a positive correlation between serum and glucocorticoid-regulated protein kinase 3 and β-catenin expression in breast cancer stem cells (r=0.318, P < 0.05). Serum and glucocorticoid-regulated protein kinase 3 may play an important role in the occurrence and development of breast cancer by regulation of Wnt/β-catenin pathway.

Key words: neoplastic stem cells, breast neoplasms, protein kinases, beta Catenin

CLC Number:

R394.2

Xu Hai. Expression of serum and glucocorticoid-regulated protein kinase 3 in breast cancer stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(40): 7061-7067.

Figures/Tables 5

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